Fig. 6

Antitumor effect in vivo, biosafety evaluation and biodistribution. a Schematic representation of the protocols of antitumor study in 4T1-bearing BALB/c nude mice. b Photographs of xenograft tumor of BALB/c nude mice after treatments with saline, FCP, CF, β-lap, CF@β-lap and HCF@β-lap for 0, 7, and 18 d, respectively. c–h Tumor volume change curves of the mice in the above treatment groups. i Relative tumor volume changes and the average tumor growth curves of the mice after administrations. j Survival rate of mice after above administrations. k H&E staining images for major tissues. l H&E, TUNEL and Ki67 immunofluorescence staining images for tumors. m The blood biochemical levels and hematological indices of mice after 24 h of administration. n Fluorescence imaging and o the corresponding quantitative analysis of the organs of tumor-bearing mice after HCF@Nr administration at 6 h. p Fluorescence imaging of the organs of tumor-bearing mice after HCF@Nr administration at 1, 2, 5, 6, 8 h. Scale bar: 50 μm for k, l. Color bar: 1 × 10⁶–3.5 × 10⁶ for n, 1 × 10⁶–5.5 × 10⁶ for p