Fig. 6

G3BP1 mediates p53 ubiquitination to regulate platinum resistance in OC. A PPI analysis revealed that G3BP1 interacts with USP10. B PPI analysis results showed that USP10 interacted with G3BP1 and p53. C and D RNA FISH showed that G3BP1 co-localised with USP10 and p53 in the cytoplasm. E and F Co-IP demonstrated that p53 protein and G3BP1 bound to each other in SKOV3/DDP and A2780/DDP cells. G CHX chase assays showed that the half-life of p53 in SKOV3/DDP cells was reduced when G3BP1 was overexpressed compared to that in the control group. H Ubiquitination assay showed that the G3BP1 overexpression enhanced the ubiquitinated p53 expression in SKOV3/DDP cells