Fig. 13

A Schematic illustration of the changes of immune cells in TIME with the treatment of LDH NPs. B LDH NPs inhibit tumor cell proliferation by interfering with autophagy. i: The expression of LC3B (left) and P62 (right) proteins in CT26 cells treated with LDH NPs (400 μg mL^–1) or CQ (10 μM). The nucleus was stained by DAPI (blue). ii: The levels of LC3B and P62 mRNA in CT26 cells-treated with LDH NPs (low, 200 μg mL^–1; high, 400 μg mL^–1) for 24 h. iii: The levels of LC3B and P62 proteins in CT26 cells-treated with LDH NPs for 24 h were detected by Western blot. C Antitumor efficacy of LDH NPs against colon tumor and melanoma. D i: The level of Trp2-specific CD3 + CD8 + IFN-γ + T cells in splenocytes (n = 3). ii: The level of Trp2-specific CD3 + CD4 + IFN-γ + T cells in splenocytes (n = 3). iii: The level of Trp2-specific CD3 + CD4 + IL-4 + T cells in splenocytes (n = 3). iv: The level of CD3 + CD8 + effector T cells in tumor (n = 3). vi: The level of CD3 + CD4 + T helper cells in tumor (n = 3). Data are means ± SEM.