Fig. 4
From: The application of nanoparticles-based ferroptosis, pyroptosis and autophagy in cancer immunotherapy
Copyright 2022, WILEY publishing group
A Fabrication of the siProminin2@PSN-FeNP B Illustration of ferroptosis induced by siProminin2@PSN-FeNP and CRT exposure and HMGB1 secretion in 4T1 tumor cells, following by CLSM (scale bars = 20 µm) G1: Control; G2: Oxaliplatin; G3: FeNP; G4: PSN-FeNP; G5: siProminini2@PSN-FeNP. C Number of 4T1 tumor cells-derived exosomes quantified with NTA, respectively (n = 3) and western blot assay of Prominin2, Ferritin expression in 4T1 tumor cells-derived exosomes (left); Flow cytometry analysis results of CD206 and CD80 expression in RAW 264.7 (pretreated with IL-4), respectively (n = 3) (right) G1: Control; G2: siProminin2; G3: siProminini2@PSN-FeNP; G4: PSN -FeNP; G5: iron ions. D Number of the lung metastatic foci and its quantification, respectively (n = 3). Data were presented as the mean ± SD (one-way ANOVA comparisons tests, ∏P < 0.05, ∏∏P < 0.01, ∏∏∏P < 0.001).