Fig. 9

A i: Mechanistic illustration of PNM-induced pyroptosis in tumor cells. ii: GSDME-N and cleaved caspase-3 protein expression in different groups of tumor tissues by Western blotting. B Improved antitumor effect of PNM in combination with αPD-1. i: The tumor growth curve of mice in different treatment groups (n = seven mice per group). ii: The survival curve of mice in different treatment groups (n = seven mice per group). C i: Representative immunohistochemical images of CD8 and immunofluorescence of Granzyme B in tumor sections in different groups (scale bar = 25 μm). Data are shown as the mean ± SEM (*P < 0.05; ***P < 0.001). ii: Representative immunohistochemical images of Foxp3 and immunofluorescence of CD11c in tumor sections in different groups (scale bar = 25 μm). Data are shown as the mean ± SEM (*P < 0.05;***P < 0.001).