Fig. 3
In vitro validation of click-mediated signal amplification. A The in vitro binding capacity of [68Ga]Ga-PSMA-Tz to Pd@Au-PEG-TCO was measured by ultrafiltration experiments. B The cell uptake assay of [68Ga]Ga-PSMA-Tz was performed with different procedures at 4 °C. The uptake of group I was defined as 100%. C Membrane binding and internalization of [68Ga]Ga-PSMA-Tz and [68Ga]Ga-PSMA617. D Time-dependent retention of [68Ga]Ga-PSMA-Tz in 22Rv1 cells with or without Pd@Au-PEG-TCO pre-incubation