Fig. 6

Combination of Aposomes and ICB therapy in the colon tumor models. a Tumor growth curves of CT26 colon tumor-bearing mice during treatment with DOXIL plus αPD-L1 Ab, Aposomes plus αPD-L1 Ab or αPD-L1 Ab only. Aposomes and DOXIL were injected with an equivalent 3 mg/kg DOX dose once every three days, and αPD-L1 Ab (10 mg/kg) was simultaneously administered via tail vein. b Tumor tissues stained with TUNEL of H&E after 13 days of treatment. c The percentage of cytotoxic T cells (CD45⁺CD3⁺CD8⁺) in the tumor tissues on day 13 after treatment. d Relative amount of TNF-α, IFN-γ and IL-17 in tumor supernatants on day 13 after treatment. e The percentage of splenic effector/memory CD8⁺ T cells (CD8⁺CD44⁺CD62L^low) in CR and naive mice. f Tumor growth curves in naive and CR mice rechallenged with CT26 cells. g Cytokine levels in plasma on day 20 after CR mice were rechallenged with secondary tumors compared to naive mice. Significance was determined by Tukey − Kramer post-hoc test