Fig. 1

Identification of the exosomes derived from hypoxic ADSCs. A Morphology of each group of exosomes photographed via TEM. Scale bar: 100 nm. B Size distribution of the exosomes was determined with a NanoSight LM10 instrument. C Western blot of CD9, CD81 and TSG101 in the exosomes. D Analysis of the expression of CD9, CD63 and TSG101 in the exosomes. E Exosome protein concentration in the normoxia and hypoxia groups using the BCA assay. F Uptake of the red fluorescence dye Dil labelled Norm-Exos and Hypo-Exos into HUVECs. Scale bar = 20 μm. n = 5. G Statistical evaluation of fluorescence intensities in the two groups (*p < 0.05; **p < 0.01; ***p < 0.001)