Fig. 3

miR-21 is up-regulated in exosomes derived from the hypoxic ADSCs. A Pie chart of the exosomal miRNA abundance analysis by high-throughput small RNA sequencing. The top 15 most abundant miRNAs in Hypo-ADSC-derived exosomes are colour labelled. B Top 15 most abundant miRNA in the Hypo-ADSC-Exos and ADSC-Exos. C qRT-PCR results show the 15 most abundant miRNAs in different gourps of HUVECs subjected to interference for 48 h. D The expression of miR-21 in the CM of Hypo-ADSCs treated with GW4869 was significantly decreased compared with the control group. E Expression of miR-21 in the HUVECs treated with GW4869-CM for 48 h was also significantly decreased compared to that in the HUVECs treated with Hypo-ADSC-CM. F Cell proliferation of HUVECs measured by EDU staining. Scale bar = 200 μm. n = 5. G Representative images of tube formation in HUVECs treated with PBS, Hypo-Exos, Hypo-Exos+si-miR-NC and Hypo-Exos, Hypo-Exos+si-miR-21. Scale bar = 200 μm. n = 5. H Quantitative analysis of the tube formation assay in different groups. I Extracted exosomes promoted cell migration as determined by a scratch test at 0 h, 8 h, 16 h and 24 h after treatment. n = 5. J Quantitative analysis of the migration rate of HUVECs. (Data are presented as the means ± SD; *p < 0.05; **p < 0.01; ***p < 0.001)