Fig. 5

SPRY1 knockdown increases the HUVECs proliferation, migration and angiogenesis. A, B After HUVECs were transfected with SPRY1, siRNA #1 was stimulated with hypo-ADSC-Exos for 24 h and the PI3K/AKT activation levels were determined by Western blot (A) and qRT-PCR (B). C Cell proliferation of HUVECs measured by EDU staining. Scale bar = 200 μm. n = 5. D Representative images of tube formation in HUVECs treated with PBS, Hypo-Exos+si-miR-NC and Hypo-Exos, Hypo-Exos+si-miR-SPRY1. Scale bar = 200 μm. n = 5. E Quantitative analysis of the tube formation assay in different groups. F Extracted exosomes promoted cell migration as determined by a scratch test at 0 h, 8 h, 16 h and 24 h after treatment. n = 5. G Quantitative analysis of the migration rate of HUVECs. (Data are presented as the means ± SD; *p < 0.05; **p < 0.01; ***p < 0.001)