Fig. 4

Quercetin performed anti-inflammation of macrophages under periodontitis microenvironment. A, B Proliferation results of RAW264.7 cells incubated with quercetin for 1 day under physiological and periodontitis environment through CCK-8 assay. C–F Effect of quercetin on the expression of inflammatory genes (IL-1β, IL-6, iNOS and TNF-α) of RAW264.7 through qRT-PCR. G–J Effect of quercetin on the expression of inflammatory cytokines (IL-1β, IL-6, iNOS and TNF-α) of RAW264.7 through Elisa tests. K Schematic of the pharmacological mechanism of quercetin on macrophages under periodontitis microenvironment. (^#p < 0.05, ^##p < 0.01 and ^###p < 0.001 compared to Pg.LPS (-) + Quercetin (-) group, ^*p < 0.05, ^**p < 0.01 and ^***p < 0.001 compared to Pg.LPS ( +) + Quercetin (-) group; Data are represented as the mean ± SEM, n = 3)