Fig. 5

Quercetin hindered the inflammatory reaction in Pg.LPS-stimulated macrophages through decreasing the expression of miR-21a-5p. A Heatmaps of differential genes between Pg.LPS ( +) + Quercetin (-) and Pg.LPS ( +) + Quercetin ( +). B Gene expression of miR-21a-5p among Pg.LPS (-) + Quercetin (-), Pg.LPS ( +) + Quercetin (-) and Pg.LPS ( +) + Quercetin ( +) groups through qRT-PCR. C, D Western blot and its quantitative analysis of p-P65 following incubation of macrophages with quercetin for 1 day. E The expression of inflammatory genes (IL-1β, IL-6, iNOS and TNF-α) through qRT-PCR in macrophages transfected with miR-21a-5p mimic NC and miR-21a-5p mimic. F–I The expression of inflammatory proteins (IL-1β, IL-6, iNOS and TNF-α) and p-P65 through western blot in RAW264.7 transfected with miR-21a-5p mimic NC and miR-21a-5p mimic. (In Fig. 5B–D, ^#p < 0.05, ^##p < 0.01 and ^###p < 0.001 compared to Pg.LPS (-) + Quercetin (-) group and ^*p < 0.05, ^**p < 0.01 and ^***p < 0.001 compared to Pg.LPS ( +) + Quercetin (-) group; In Fig.  5E–I, *p < 0.05, **p < 0.01 and ***p < 0.001; Data are represented as the mean ± SEM, n = 3)