Fig. 2

Intracellular endocytosis, cytotoxicity and in vitro antitumor effect of MF@DiI. (A) CLSM images of the intracellular endocytosis of MF@DiI (scale bar: 50 μm). (B) FCM analysis of the intracellular endocytosis of MF@DiI. Viability of (C) Hepa1-6 cells and (D) HUVECs after incubation with different concentrations of MSN, MF, MSN@SOR, and MF@SOR for 12 h (n = 3). (E) CLSM images of live and dead hepa1-6 cells after incubation with different NPs and (F) the corresponding FL percentage (scale bars: 100 μm). In panels (C) and (D), the data are presented as the mean ± SD, and the p values were calculated by ANOVA. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001, ns, not significant