Fig. 5

Analysis of immunomodulation in the primary tumor model. DC maturation (CD11c⁺ CD80⁺ CD86⁺) in the (A) spleens and (B) TDLNs. Quantitative analysis of DC maturation (CD11c⁺ CD80⁺ CD86⁺) in the (F) spleens and (G) TDLNs (n = 3). FCM analysis of CD3⁺CD8⁺ T cells in the (C) spleens, (D) TDLNs, and (E) tumors, and (H, I, and J) quantitative analysis (n = 3). (K) IF images of CD8⁺ T cells in hepa1-6 tumors after different treatments (scale bar: 50 μm). (L) IHC of Foxp3⁺ expression in tumor tissues (scale bar: 50 μm). In panels (F), (G), (H), (I) and (J), the data are presented as the mean ± SD. All p values were calculated by ANOVA. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001, ns, not significant