Fig. 6

In vivo antitumor effect and MRI. (A) Schematic illustration of the experimental design of the primary tumor models (created with BioRender.com). (B) Time-dependent relative tumor volume growth curves in the different groups (n = 5). (C) Time-dependent body weight curves of mice in different groups (n = 5). (D) Survival curves of mice in different groups (n = 5). (E) Representative images of TUNEL, PCNA and H&E analysis in each group (scale bar: 50 μm). Semiquantitative FL intensity analysis of (F) PCNA and (G) TUNEL staining (n = 3). (H) H&E staining of the major organs in each group after various treatments (scale bar: 100 μm). Representative (I) digital photos and (J) weights of dissected tumors from different groups at the end of treatment (n = 5). (K) T1 MRI of subcutaneous models at different time points. (L) Corresponding change mean signal intensity in the tumor regions in the subcutaneous models at different time intervals (n = 3). In panels (B), (C), (D), (F), (G), (J) and (L), the data are presented as the mean ± SD. All p values were calculated by ANOVA. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001, ns, not significant