Fig. 7

Long-term immune effects in the recurrent and metastatic models. (A) Schematic illustration of the animal experimental design for the recurrent tumor model (created with BioRender.com). (B) Body weight changes in recurrent tumor-bearing mice (n = 3). (C) Recurrent relative tumor volume growth curves for each group (n = 3). (D) Weights of the excised recurrent tumors in each group (n = 3). (E) FCM analysis of CD3⁺CD8⁺ T cells in the recurrent tumors in each group and (F) quantitative analysis. (G) Schematic illustration of the animal experimental design for the lung metastasis tumor model (created with BioRender.com). (H) Representative photographs and H&E staining images of lung tissue from mice. In panels (B), (C) and (D), the data are presented as the mean ± SD. All p values were calculated by ANOVA. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001, ns, not significant