Fig. 3
Intracellular integrity, uptake mechanisms and distribution of NCG. A Schematic diagram of FRET technology. Blue fluorescence corresponds to DAPI, green fluorescence signifies donor C6, and red fluorescence denotes acceptor DiI, scar bar: 20 μm. B, C The effects of various endocytosis inhibitors on the uptake of NC, NG, and NCG by Caco-2 cells and WRL-68 cells are examined. Statistical analysis of fluorescence intensity for NC, NG, and NCG groups in Caco-2 cells and WRL-68 cells under different endocytosis inhibitors is provided (n = 6). Data are presented as mean ± SD, ***p < 0.001 compared to the Control group. Scale bar: 50 μm. D The co-localization of NCG with lysosomes (Lyso), endoplasmic reticulum (ER), and mitochondria (Mito) in Caco-2 and WRL-68 was visualized using appropriate tracking dyes. Scale bar: 20 μm