Fig. 4

MoS₂ NDs improved collateral formation in diabetic mice. (A) Illustration of time points of MoS₂ NDs injection and laser Doppler imaging in diabetic mice. (B) Representative H&E images of muscles harvested at the indicated time with PBS and MoS₂ NDs. (C) Quantification of the regenerating area at different time points, n = 5, and P values were calculated by two-way ANOVA with Bonferroni post-hoc test. (D) Quantification of the necrotic area at indicated times from H&E staining, n = 5, and P values were calculated by two-way ANOVA with Bonferroni post-hoc test. (E) Representative images of hindlimb blood perfusion with PBS and MoS₂ NDs at indicated times. (F) Quantification of hindlimb blood perfusion at the indicated times, n = 5, and P values were calculated by two-way ANOVA with Bonferroni post-hoc test. (G) Representative DHE staining images and quantification of DHE⁺ area on day 7, n = 9, P values were calculated by t-test. (H) Representative CD31⁺/α-SMA⁺ immunofluorescent images and quantification of CD31⁺ area and α-SMA area on transverse cross sections on day 7, n = 9, and P values were calculated by t-test. All data are presented as the mean ± SD.