Fig. 2
In vitro cytotoxicity of SF/MXene hydrogel and electrostimulation of NSCs on SF/MXene hydrogel with different voltages. A Live/dead cellular staining of the NSCs seeded on the surface of SF/MXene hydrogels containing different concentrations of MXene on day 3. The control group cells were directly seeded onto the surface of the tissue culture plate. Live cells were stained green, and dead cells were stained red. B 3D view of NSCs in SF/MXene hydrogels containing different concentrations of MXene on day 3. C Statistics of adherent cells in each group on day 5. D Proliferation ability of NSCs after being cultured on the surface of SF/MXene-1% hydrogels containing different concentrations of MXene for 1, 3, and 5 days. E Proliferation of NSCs on SF hydrogel and F SF/MXene hydrogel on 1, 3, and 5 d after ES with different voltages. G The gene expression levels of neurons (β-tubulin III) and H astrocyte (GFAP) were analyzed on day 5. I Live/dead cellular staining of the NSCs in SF, SF/MXene, SF/MXene + ES (300 mV cm−1) groups on day 5. (± SD, n = 3; *P < 0.05; **P < 0.01; ***P < 0.001)