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Fig. 3 | Journal of Nanobiotechnology

Fig. 3

From: A versatile engineered extracellular vesicle platform simultaneously targeting and eliminating senescent stromal cells and tumor cells to promote tumor regression

Fig. 3

uPA-sEVs-siSrc induce apoptosis of senescent stromal cells in vitro. (A) Schematic illustration describing the experimental design in vitro. (B) mRNA expression of Src in proliferative and senescent HFF-1 and HMEC-1 cells with different treatments determined by RT-qPCR respectively. (C) Protein levels of p-Src and Src in proliferative and senescent HFF-1 and HMEC-1 cells with different treatments determined by western blotting. (D) Cell viability analysis of proliferative and senescent HFF-1 and HMEC-1 cells with different treatment for 48 h. (E) Caspase 3 activity of senescent HFF-1 and HMEC-1 cells with different treatments. (F) Flow cytometric examination and quantitative analysis of senescent HFF-1 and HMEC-1 cells apoptosis with different treatments. Data are displayed as the mean ± SD (n = 3). **p<0.01; ***p<0.001; ****p<0.0001

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Journal of Nanobiotechnology

ISSN: 1477-3155

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