Fig. 3

Polarized macrophages by BRT-O scaffolds promoted the osteogenic differentiation of BMSCSs in vitro. (A) CCK-8 analysis of BMSCSs after 1, 3 and 7 days of co-culture with different conditioned media (CM). (B) The qRT-PCR analysis of the relative mRNA expression levels of RUNX2 and BMP2 in BMSCSs on day seven. (C) Western blot results for the RUNX2 expression levels in BMSCSs on day seven. (D) The transwell assay of BMSCSs induced by different CM. (E) The quantification analysis of cell migration. (F) Representative images of the wound healing capacities of BMSCSs after co-culture with different CM for the indicated time periods. (G) Quantitative analysis of the wound healing. (H) ARS staining of BMSCSs on day 21. (I) Semi-quantification analysis of ARS. The data were expressed as mean ± standard deviation (SD) (n = 3). Ns, no significance; ^**p < 0.01 vs. the control group; ^***p < 0.001 vs. the control group; ^#p < 0.05 vs. the BRT-R group; ^###p < 0.01 vs. the BRT-R group. Scale bars: D, 100 μm; F, 200 μm; H, 100 μm