Fig. 2

DEGs and Enrichment analysis of Monocytes. (a) Monocyte subsets from the control and treated groups are plotted using UMAP. (b) Population differences of monocyte subsets from untreated control and treated group. (c) Volcano plot showed significant gene expression from both the control and treated groups. (d) Bar plot graph showed differentially expressed genes (DEGs) from monocyte subsets. (e) Gene ontology enrichment analysis showed up and downregulation genes involved in various functional annotation of pathway list. (f) Gene set enrichment analysis (GSEA) of significant genes from monocyte subsets. (g) Types of metallothionein genes are mostly involved in metal ion –cellular responses. (h) Illustration of monocytes and their subsets with their up and down regulated genes involved in proposed functional pathway. For all DEG analyses, a significant change was defined as an adjusted p-value (Benjamini-Hochberg analysis) less than 0.05 and a fold change over two times. For all GO and GESA analyses, an adjusted p-value (Benjamini-Hochberg analysis) of less than 0.05 was used to identify significant enrichment