Fig. 3

DEGs and Enrichment analysis of B cells. (a) UMAP is used to plot B cell subsets from the control and treated groups. (b) Population differences of B cells subsets from untreated control and AgNP treated group. (c) Significant gene expression from both the control and treated groups was visible on the volcano plot. (d) Differentially expressed genes (DEGs) from B cell subsets were displayed in a barplot graph. (e) Gene ontology enrichment analysis for B cells performed and showed for both up and downregulated genes. (f) For GSEA analysis, targeted GOs from B cell subsets were targeted and showed. (g) naïve and memory B cells and their subsets are depicted, along with the up and down regulated genes involved in the suggested functional pathway. For all DEG analyses, a significant change was defined as an adjusted p-value (Benjamini-Hochberg analysis) less than 0.05 and a fold change over two times. For all GO and GESA analyses, an adjusted p-value (Benjamini-Hochberg analysis) of less than 0.05 was used to identify significant enrichment