Table 1 Synthesis methods and conditions of tFNAs
From: Tetrahedral framework nucleic acids for improving wound healing
Synthesis method | Experimental method | Specific sequence (from 5’ to 3’) | Number of bases per single strand | Ref |
|---|---|---|---|---|
Self-assembled | Heated at 95 °C for 2 min followed by incubation in ice bath | S1 ACA TTC CTA AGT CTG AAA CAT TAC AGC TTG CTA CAC GAG AAG AGC CGC CAT AGT A S2 SH-C6-TAT CAC CAG GCA GTT GAC AGT GTA GCA AGC TGT AAT AGA TGC GAG GGT CCA ATA C S3 -SH-C6-TCA ACT GCC TGG TGA TAA AAC GAC ACT ACG TGG GAA TCT ACT ATG GCG GCT CTT C S4 -SH-C6-TTC AGA CTT AGG AAT GTG CTT CCC ACG TAG TGT CGT TTG TAT TGG ACC CTC GCA T | 73 | [59] |
Self-assembled | Heat up to 95 °C for 10 min then cool down to 4 °C for half an hour | S1 ATTTATCACCCGCCATAGTAGACGTATCACCAG GCAGTTGAGACGAACATTCCTAAGTCTGAA S2 ACATGCGAGGGTCCAATACCGACGATTACAGC TTGCTACACGATTCAGACTTAGGAATGTTCG S3 ACTACTATGGCGGGTGATAAAACGTGTAGCAA GCTGTAATCGACGGGAAGAGCATGCCCATCC S4 ACGGTATTGGACCCTCGCATGACTCAACTGCC TGGTGATACGAGGATGGGCATGCTCTTCCCG | 63 | [60] |
Self-assembled | Heated to 95 °C for 4 min and cooled rapidly on ice for 30 min | S1 CGCGACTTAGGTCCATAATCAAGGGGCCGGTG AGATGGGAGTGAACGGGTCTGG S2 TAGCGTTAGGACAACGGAATCTCACCGGCCCC TTGATACGTGCGGGTCTGATAA S3 TTATGGACCTAAGTCGCGAGTCCAGAATAAGG AACTTTATCAGACCCGCACGTA S4 ACTCCAGACCCGTTCACTCCCTCCGTTGTCCAA CGCTAAG | 54;54; 54;40 | [61] |
Self-assembled | Denatured at 95 °C for 5 min and then cooled to 4 °C for 1 h | S1 CCAGGCAGTTGAGACGAACATTCCTAAGTCTG AAATTTATCACCCGCCATAGTAGACGTATCA S2 CTTGCTACACGATTCAGACTTAGGAATGTTCGA CATGCGA GGGTCCAATACCGACGATTACAG S3 GGTGATAAAACGTGTAGCAAGCTGTAATCGAC GGGAAGA GCATGCCCATCCACTACTATGGCG S4 CCTCGCATGACTCAACTGCCTGGTGATACGAG GATGGGCA TGCTCTTCCCGACGGTATTGGAC | 65;64 64;64 | [62] |
DNA origami | Annealed at 95 °C for 30 s, followed by a temperature decrease of 10 min/ °C until 65 °C, afterward a decrease of 20 min/ °C until 20 °C | S1 GACTAAAGGAGGATATCATCGGACAG S2 CGCATAACGGTCGAAAGGCCGATCGTCAC S3 TATTCCGATATTAGCTTTAGCTT S4 AGTTTCCATTAATTGTATCGGTATAGCGGGCT TTT | 26;29 23;35 | [63] |
Enzymatic assembly | Heated to 90 °C for 5 min and then cooled down to 4 °C for 10 min | S1 ACACTACGTCAGAACAGCTTGCATCACTGGTC ACCAGAGTA S2 ACGAGCGAGTTGATGTGATGCAAGCTGAATGC GAGGGTCCT S3 TCAACTCGCTCGTAACTACACTGTGCAATACT CTGGTGACC S4 TCTGACGTAGTGTATGCACAGTGTAGTAAGGA CCCTCGCAT | 41 | [64] |
Enzymatic assembly | Heated to 70 °C for 5 min and then cooled down to 4 °C for 1 h | S1 AGGCAGTTGAGACGAACATTCCTAAGTCTGAA ATTTATCACCCGCCATAGTAGACGTATCACC S2 TATTTTGTTATGTGTTATGTGTTATATTCAGACTT AGGAATGTTCGACATGCGAGGGTCCAATACCG S3 CCCCCCCTAACCCCCCCTAACCCCCCCTAACC CCCCCATACTGTAATCGACGGGAAGAGCATGCCC ATCCACTACTATGGCGGGTGATAAA S4 CCTCGCATGACTCAACTGCCTGGTGATACGAG GATGGGCATGCTCTTCCCGACGGTATTGGAC | 63;67 91;63 | [65] |