Fig. 2

A After different treatments, the NO level in 4T1 cells was detected using a DAF-FM DA fluorescent probe by CLSM. B The NO level in 4T1 cells was detected using FCM. C Immunohistochemical examination of 3-NT (brown) in the tumor tissues. D Collagen I immunofluorescent staining of tumors (Red, collagen I; Blue, DAPI). E The increased expressions of MMP-1 and MMP-2 were detected by Western blotting. F Quantitative analysis of MMP-1 and MMP-2 variations. G After different treatments for 6 h, the NO level in vivo was detected using immunofluorescent staining (blue, cells; green, NO). The experiments were repeated thrice independently. ANOVA with Tukey’s post-hoc test. *p < 0.05, **p < 0.01, and ***p < 0.001