Fig. 5

IVIS spectrum CT/MRI/PAI multi-modality imaging in vitro and in vivo. A In vivo NIR fluorescence images of tumors in 4T1 tumor-bearing mice after intravenous injection of DNMF/PLGA NPs at different time points. B In vitro PAI images and PAI values of DNMF/PLGA NPs at different concentrations. C ICP assay of tumors showing the Fe content in the different time points (pre, 1 h, 6 h) after intravenous administration in mice. D ICP assay of tumors showing the Mn content at different time points (pre, 1 h, 6 h) after intravenous administration in mice. E The average photoacoustic intensity at tumor regions after an intravenous injection of NPs at different time intervals. F In vivo PAI images of tumors in 4T1 tumor-bearing mice after intravenous injection of DNMF/PLGA NPs at different time intervals. G In vitro MRI T1 contrast images, mappings, and R1* value of DNMF/PLGA at different nanoparticle concentrations (0, 0.625, 1.25, 2.5, 5, and 10 mg/mL). H In vivo MRI T1 images of tumors were obtained at 0 and 6 h after an injection of DNMF/PLGA NPs. Data are presented as the means ± SD. Two-way ANOVA with repeated measures with Tukey’s post-hoc test. *p < 0.05