Fig. 5
From: Young osteocyte-derived extracellular vesicles facilitate osteogenesis by transferring tropomyosin-1
Proteomic profiling of YO-EVs and SO-EVs for comparative analysis. Heatmap showing the differentially enriched proteins (absolute fold change ≥ 1.5, P < 0.05) between YO-EVs and SO-EVs (A). n = 3 per group. Relative protein level of TPM1 in YO-EVs and SO-EVs (B). n = 3 per group. qRT−PCR analysis of Tpm1 expression levels in bone matrix and bone marrow samples from young and elderly mice, respectively (C). n = 3 per group. The inhibitory efficiency of siRNAs targeting Tpm1 was verified by qRT−PCR analysis (D). n = 3 per group. Phalloidin (Phal) immunofluorescence staining images (E) and the intensity of the Phal+ area (F) of BMSCs treated with Solvent, YO-EVs, or SO-EVs. Scale bar: 20 μm. n = 5 per group. ARS staining (G; red), ORO staining (J; red), quantification of the percentages of ARS+ area (H), ORO+ area (K), qRT−PCR analysis of Runx2 (I) and Pparg (L) of BMSCs treated with Solvent, YOsi−Control-EVs, or YOsi−Tpm1-EVs under osteogenic or adipogenic induction. Scale bar: 50 μm. n = 5 or 3 (qRT−PCR analysis) per group. PBS treatment was used in the solvent groups. * P < 0.05, ** P < 0.01, **** P < 0.0001