Fig. 6
From: Young osteocyte-derived extracellular vesicles facilitate osteogenesis by transferring tropomyosin-1
Contribution of TPM1 to YO-EV-promoted bone formation. µCT-reconstructed images of femurs from mice treated with Solvent, YOsi−Control-EVs, or YOsi−Tpm1-EVs (A). Scale bar: 1 mm. Quantification of Tb. BV/TV, Tb. N, Tb. Th, Tb. Sp of trabecular bone (B), and Ct. Th of cortical bone (C). Three-point bending measurement of the femoral ultimate load values from different treated mice (D). OCN immunohistochemical staining images (E), the number of OCN+ (brown) osteoblasts (N. OBs) on the trabecular bone surface (F), and the concentration of OCN in serum (G). PLIN immunofluorescence staining images (H), the number of PLIN+ (red) adipocytes in bone marrow (N. AdCs per mm2; I). Scale bar: 50 μm (in E) or 100 μm (in H). n = 5 per group. BS: bone surface, Ar: area. PBS treatment was used in the solvent groups. * P < 0.05, ** P < 0.01