Fig. 6
From: Carbon quantum dots of ginsenoside Rb1 for application in a mouse model of intracerebral Hemorrhage
RBCQDs reduce brain tissue iron content and alleviate oxidative stress damage after ICH. (a) Experimental timeline for in vivo mouse studies. Colorimetric assay to detect Fe2+ (b) and Fe3+ (c) levels in brain tissue (n = 6/group, one-way ANOVA). (d) DCFH- DA probe assay to measure ROS levels in mouse brain tissue cells (n = 6/group, one-way ANOVA). (e) Immunofluorescence staining images showing the content of MDA and cleaved caspase-3 in neurons of brain tissue. Nissl staining marks neurons, FITC labels MDA, TRITC labels cleaved caspase-3, and DAPI marks cell nuclei.The scale bar length in (e) was 20 μm. Relative fluorescence intensity of MDA (f) and cleaved caspase-3 (g) in neurons of brain tissue (n = 6/group, one-way ANOVA). Immunoblot bands (h) and quantitative analysis showing the content of MDA (i) and cleaved caspase-3 (j) in neurons of brain tissue in different treatment groups (n = 6/group, one-way ANOVA)