Fig. 4

The ferroptosis induced by OXA@Fn in GL261/TR cells. A Effect of OXA@Fn on the viability of GL261/TR cells in the presence of IFN-γ (10 ng/ml), DFO (60 μg/ml) and Fer-1 (3 μg/ml). B The effect of OXA@Fn on DNA damage (marked with γ-H2AX antibody) and the live/death GL261/TR cells. C The effect of OXA@Fn on the cloning formation of GL261/TR cells. D The effect of OXA@Fn on Fe²⁺ level in GL261/TR cells. E The effect of OXA@Fn on GSH level in GL261/TR cells. F The effect of OXA@Fn on the expression of GPX4 in GL261/TR cells in the presence of IFN-γ (10 ng/ml), DFO (60 μg/ml) and Fer-1 (3 μg/ml). G The effect of OXA@Fn on LPO (marked with BODIPY-C11, green color) and ROS (marked with DCF, green color) level in GL261/TR cells. H The effect of OXA@Fn on the expression of FPN1 in GL261/TR cells. The OXA@Fn concentration was equivalent to 45 μg Fn/ml. (n = 3, mean ± SD, *P < 0.05, **P < 0.01)