Fig. 3

a Confocal images of fresh mouse brain section incubated with MyL-1 compound (20 μM) for 30 min, the co-stained or immunostained with other brain parenchymal marker, including tubulin for neuronal cytosol, MAP2 for synapse, NeuN for neuronal nucleus, GFAP for astrocytes and Lectin for brain capillaries, lower row are magnified micrographs from selected region from upper row. Cell nucleus were marked by DAPI. b Confocal images of fresh mouse brain section incubated with MyL-1 compound (20 μM) for 30 min, then co-stained with FluoroMyelinTM Green, magnified images showed a high overlap degree. c Colocalization analysis indicated by Pearson Coefficiency Rr from the above co-stain experiments (n = 10–20 images from three independent experiments)