Fig. 5

a Mouse brain section (corpus callosum region) incubated with MyL-1 compound (20 μM) for 30 min, and immunofluorescently stained with MBP (ATTO 655 STED secondary antibody, STED depletion = 775 nm), the nucleus is marked by DAPI. b Magnified two-color STED imaging showed myelin and MBP distribution along a single axon. c MyL-1 and MBP fluorescence intensity analysis along a selected region from b at a single axon. d Quantification of myelin (MyL-1) and MBP node distance along axons (n = 10–20 images from three independent experiments. Statistical analysis: student t-test, NS = No Significance)