Fig. 6

CeONZs permitted survival of dissociated hESCs. A Temporal analysis of ROS intensity in H9 hESCs passaged with E8 only and E8 plus Y27632. *Means 1 h vs 0 h in E8 only, ^#means E8 plus Y27632 vs E8 only. B Phase images of H9 cultured in E8 only, E8 plus Y27632, E8 plus 1.5 μM or 6 μM CeONZs for 24 h after cell dissociation (Scale bar = 75 μm). C Cell viability of H9 hESCs after seeding 1 day and 2 days. H9 cells were cultured in E8 only, E8 plus Y27632, and E8 plus 1.5 μM or 6 μM CeONZs for 24 h. D, E Flow cytometry analysis showing ROS levels of H9 cultured in E8 only, E8 plus Y27632, E8 plus 1.5 μM, or 6 μM CeONZs for 24 h after dissociation. F TUNEL assay of apoptotic H9 hESCs treated with E8, E8 plus Y27632, or E8 plus indicated concentrations of CeONZs for 24 h after dissociation. G, H Representative alkaline phosphatase (ALP) staining of H9 hESCs passaged using E8 only, E8 plus Y27632, and E8 plus 1.5 μM or 6 μM CeONZs. The number of colonies was counted on day 7 after plating. *Means the comparison of the total number of colonies. Data are depicted as means ± SEM (n = 3), and statistical significance was determined by one-way ANOVA followed by Tukey’s post hoc test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. ^#P < 0.05,  ^####P < 0.0001