Fig. 4

In vivo therapeutic assessment of AA/γCD in treatment of mice ALI. (A) Experiment schedule. LPS (10 mg/kg) was intratracheally injected to induce ALI, and AA/γCD was intravenously administered 4 h later. Normal mice exposed to saline challenge served as the control. (B) Percentage of body-weight change of mice at 24 h after LPS treatment. (C) Lung index. (D) MPO activity of lung homogenates and BALF. (E) TNF-α and IL10 in lung homogenates, BALF, and plasma were assessed by ELISA. (F) mRNA levels of TNF-α, IL-1α, IL-1β, IL-6, IL-10 in lung homogenates determined by real-time qPCR. (G) H&E-stained lung sections. Arrows indicate: 1, thickening of the alveolar and infiltration of inflammatory cells; 2, hemorrhage; 3, interstitial edema. Statistical comparison between control and other groups are marked by *, with *, **, and *** representing  < 0.05, P < 0.01, and P < 0.001, respectively. Between-group comparisons with LPS are marked by #, with #, ##, and ### representing P < 0.05, P < 0.01, and P < 0.001, respectively