Fig. 2

Schematic illustration of the enzymes’ function, including nicking endonuclease Nt.BbvCI, trypsin, and trypsin inhibitor. (A) Schematic illustration of the activity of nicking endonuclease, trypsin, and trypsin inhibitor; (B) Fluorescence curves of experiments demonstrating the activity of nicking endonuclease and trypsin; (C) Fluorescence curves of experiments with various concentrations of trypsin; (D) Fluorescence curves of experiments demonstrating the activity of trypsin inhibitor; (E) Fluorescence curves of experiments with various concentrations of trypsin inhibitors; (F) Experimental process and (G) Fluorescence curves of experiments demonstrating the breakthrough of trypsin inhibitors. Ordaining fluorescent results of fluorescently FAM-labeled strand as 100% and Reporter duplex as 0%. The nucleic acid sequence and concentration of this part’s experiments are shown in Table S1