Fig. 3

Schematic illustration of principle validation. (A) Schematic illustration of the amplification effect of Fuel strand; (B) Fluorescence results of experiments with various concentrations of Fuel strand; (C) Schematic illustration and fluorescence curves of three runs of simplified OR gate and complete OR gate; (D) Schematic illustration of the upstream YES gate in YES-OR gate; (E) Fluorescent curves of three runs of YES-OR gate; (F) Experimental process, critical points and (G) fluorescent curves of experiments directly validating the function of Nt.Alwi. Ordaining fluorescent results of Output + Reporter as 100% and Reporter duplex as 0%. The nucleic acid sequence and concentration are shown in Table S2. Original fluorescence curves and processing of Simple OR gate are demonstrated in Figure S4