Fig. 2

Cellular uptake of TK-MLP NPs. (A&B) Representative fluorescence images and quantitation of activated HUVECs and RAW264.7 cells 4 h after incubation with LP, MLP, TK-LP NPs, and TK-MLP NPs (red). Green represents the Lyso Tracker. Scale bar = 20 μm. (C)The schematic diagram showed that HUVECs were co-cultured with RAW264.7 cells in a transwell system for simulating plaque in vitro. (D&E) Phagocytosis and quantitation of LP NPsand TK-MLP NPs in activated HUVECs and RAW264.7 cells in transwell. BF indicates bright field. Scale bar = 20 μm. n = 3, ^##P < 0.01, ^###P < 0.001.