Fig. 5
Regulatory effects of PTMH on the microenvironment in vivo. (A) Fluorescent images and (B) statistical analysis of PCNA (red) and DAPI (blue) in mouse diabetic wounds tissue. (Scale bar: 100 μm) (C) Fluorescent images and (D) statistical analysis of CD31 (red) and DAPI (blue) in mouse diabetic wounds tissue. (Scale bar: 100 μm) (E) Fluorescent images and (F) statistical analysis of DHE (red) and DAPI (blue) on days 3 and 14 post-treatment wound tissue. (G) The distribution of cells in wound tissue obtained from different groups on days 3 and 14 post-treatment, stained with rat anti-mouse CD11b-Brilliant Violet 650™, LY6G-FITC, and CD86-Brilliant Violet 421™ (1:200). (H) Statistical analysis of CD86-Brilliant Violet 421TM-positive cells. (I) The concentrations of TNF-α, (J) IL-6, (K) IL-1β, (L) IL-10, (M) VEGF, (N) SOD and (O) MPO in wound tissue were determined using ELISA. Data in B, D, F, H and I–O represent the mean ± standard deviation (n = 5). *p < 0.05; **p < 0.01; one-way ANOVA. SA, sodium alginate; PNIPAM, poly(N-isopropylacrylamide); PTMH, programmed time-released multifunctional hydrogel; PCNA, proliferation cell nuclear antigen; DHE, dihydroethidium; CD, cluster of differentiation; ELISA, enzyme-linked immunosorbent assay; TNF-α, tumor necrosis factor-α; IL, interleukin; VEGF, vascular endothelial growth factor; SOD, superoxide dismutase; MPO, myeloperoxidase; ANOVA, analysis of variance; DAPI, 4’,6-diamidino-2-phenylindole; SSC-A, side scatter area