Fig. 1

Chemical modifications of microRNAs and functional validation. a Design schematic of three chemical modification approaches for miR-126-3p. b Stability validation of miR-126-3p after three different chemical modifications: The unmodified miR-126-3p (miR-126) and the modified versions (126-mod1, 126-mod2, 126-mod3) were transfected into DPSCs (Dental Pulp Stem Cells), and the level of miR-126 is assessed at different time points (24, 48, 72, 96 h) using qPCR. c Functional validation of chemically modified microRNAs: WB analysis of the expression of angiogenesis-related proteins after transfection (at 96 h post-transfection) of different versions of miR-126 into DPSCs. d The presentation of related proteins in (c) was quantitatively analyzed. e qPCR analysis of the expression of angiogenesis-related genes after transfection of different versions of miR-126 into DPSCs. Data include the mean ± SD, statistical analysis: \(\hbox {n}=3\), \(*p<0.05, **p<0.01, ***p<0.001\), NS, no significance