Fig. 3

Evaluation of the efficacy and safety of Gd-HPMA12 on cells. A: CLSM images of 4T1 cells treated with R6G-Gd-HPMA12 for 2.0 h at 37 °C. PBS was used as a control. Green fluorescence: Actin-Tracker for cytoskeleton; red fluorescence: R6G for Gd-HPMA12; blue fluorescence: DAPI for nuclei. B, C: R6G fluorescence distributions of 4T1 cells incubated wit PBS or R6G-Gd-HPMA12 B, and the corresponding quantitative analysis C determined by flow cytometry, showing the level of cellular internalization of the R6G-loaded nanoparticles. Mean ± SD, n = 3. *** P < 0.001. D: The internalized amount of Gd-HPMA12 into 4T1 cells for various durations (0.25, 0.50, 1.0, or 2.0 h) measured by ICP