Fig. 1
From: Macrophages as carriers of boron carbide nanoparticles dedicated to boron neutron capture therapy
A Scheme of obtaining unpolarized (M0) bone marrow-derived macrophages (BMDM) and polarized to the M1 and M2 phenotypes. B Scheme of flow cytometry analysis showing phenotypic characterization of the BMDM obtained after 8 days of culture. Among the CD11b+F4/80+ population, M1 (CD206−) and M2 (CD206+) macrophages were identified in cell culture treated with LPS and IFN-γ, or with IL-4, respectively. Additionally, the expression of CD40 and CD86 molecules on the surface of BMDM was determined. C Effect of boron carbide preparations (B4C 1, B4C 2) on the viability of M0, M1 and M2 macrophages after 72 h of exposure determined by the MTT assay. The graphs represent the percentage of viable cells relative to control cells (Control = 100%). D Concentrations of B4C 1 and B4C 2 causing 50% inhibition of cell proliferation (IC50) calculated for each macrophage type. Results are expressed as means ± SD calculated for two independent experiments performed in triplicate. Differences between groups were calculated using two-way ANOVA followed by Tukey’s multiple comparison post-hoc test (*p < 0.05; **p < 0.01)