Fig. 2
From: Macrophages as carriers of boron carbide nanoparticles dedicated to boron neutron capture therapy
Evaluation of apoptosis induction in phagocytic cells by boron carbide preparations (B4C 1, B4C 2) using the Annexin V binding assay. A Scheme of flow cytometry analysis showing the method of determining cells from lines in early apoptosis (AnV+PI−) and late apoptosis (AnV+PI+) after exposure to boron carbide. B Percentage of apoptotic cells (early and late apoptotic cells) after 24 and 72 h of exposure to boron carbide preparations in RAW264.7, J774A.1 and JAWS II cells. C Scheme of flow cytometry analysis showing the method of determining F4/80+ bone marrow-derived macrophages in early apoptosis (AnV+PI−) and late apoptosis (AnV+PI+) after exposure to boron carbide. D Percentage of apoptotic cells (early and late apoptotic cells) after 24 and 72 h of exposure to boron carbide preparations in BMDM (M0, M1 and M2) populations. The graphs represent the percentage of apoptotic cells after deducting the percentage of apoptotic cells in the untreated control. ‘ND’ means no difference was detected between treated cells and untreated control. Results are expressed as mean + SD calculated for two independent experiments performed in triplicate. The differences between groups were calculated using the two-way ANOVA followed by Tukey’s multiple comparison post-hoc test (*p < 0.05; **p < 0.01; ***p < 0.001)