Fig. 4

(A) Confocal microscopy images of FITC-loaded NPs uptake by untreated RAW cells or RAW cells precultured with LPS for 1, 2 and 3 h (scale bar: 50 μm). (B) The FCM results of the FITC signal to demonstrate the uptake efficiency of NPs at 1 and 3 h, stained with FITC and F4/80. (C) The FITC-positive percentage of cells cultured with FITC-loaded NPs after 1 and 3 h. (D) The FCM results of BMMs cultured with normal medium and medium cotaining Fu (15 and 30 µg/mL, repectively) or HAZ@Fu NPs (80 µg/mL) for 24 h, stained with the M2 marker CD206, and macrophage markers CD11b (gated by F4/80). (E) FCM results of BMMs cultured with normal medium and medium containing LPS (100 ng/mL), LPS + Fu or LPS + HAZ@Fu NPs stained with the M1 marker CD86 and macrophage marker CD11b (gated by F4/80). (F) Ratio of BMMs stained with M2 marker CD206. (G) Ratio of M1 phenotype BMMs stained with M1 marker CD86