Fig. 6

(A) Colocalization of ZIF@FITC NPs with lysosomes. RAW 264.7 cells were incubated with 60 µg/mL FITC-loaded NPs for 4 h. Then the slides were rinsed with PBS and treated with LysoTracker Red for 5 min at 37 °C. Images were immediately captured from live cell (scale bar: 10 μm). (B) Colocalization of ZIF@FITC NPs with lysosomes (scale bar: 10 μm). (C) Colocalization of HAZ NPs with intracellular MRSA. RAW 264.7 cells were incubated with MRSA (marked by mCherry). After which extracellular bacteria were removed, the cells were then rinsed with PBS and further incubated with 80 µg/mL HAZ@FITC NPs for 3 h. Images were captured using CLSM (scale bar: 10 μm). (D) PCC was calculated using ImageJ. (E) Infected RAW 264.7 cells were incubated with Fu, ZIF, HAZ, ZIF@Fu, HAZ@Fu, and PBS for 2 and 5 h. Subsequently, all cells were lysed, and 100 µL of each lysate (10-fold dilution) was cultured on LB agar plates and incubated for 24 h. Digital images of the plates were captured. (F) Viable cell counts were calculated using three biological replicate count data (each derived from three technical replicate data). All data shown are viable counts multiplied by 100 (dilution multiple), with log10 transformed