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Fig. 3 | Journal of Nanobiotechnology

Fig. 3

From: Osteosarcoma-targeted Cu and Ce based oxide nanoplatform for NIR II fluorescence/magnetic resonance dual-mode imaging and ros cascade amplification along with immunotherapy

Fig. 3

(A) CLSM photographs of 143b cells after treated with free ICG, mCu&Ce@ICG and mCu&Ce@ICG/RGD. (B) ROS measurement images of 143b cells after incubated with mCu@ICG/RGD and mCu&Ce@ICG/RGD followed by laser irradiation and H2O2 + laser. (C) CLSM pictures of intracellular •OH production after 143b cells were treated with mCu&Ce@ICG/RGD and mCu&Ce@ICG/RGD + H2O2 + L, respectively. (D) CLSM images of membrane-anchored CRT expression in 143b cells after incubated with mCu@ICG/RGD and mCu&Ce@ICG/RGD followed by laser irradiation and H2O2 + laser. (E, F) Western-blot analysis of CRT and HMBG1 expressions in 143b cells after incubated with mCu@ICG/RGD and mCu&Ce@ICG/RGD followed by laser irradiation and H2O2 + laser. (G) Quantitative green signal intensity in cellular uptake in Fig. 3A. (H) Quantitative green signal intensity of ROS generation in Fig. 3B. The ratios of CTR/β-action (I) and HMGB1/β-action (J) in Fig. 3E. (K) live-dead cell fluorescent images, (M) Cell apoptosis-necrosis analysis and (L, N) corresponded quantitative analysis of 143b cells after treated with various formulations. **p < 0.01. Above presented data is as means with standard deviations (n = 3) (mean ± SD)

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Journal of Nanobiotechnology

ISSN: 1477-3155

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