Fig. 2

Biodistribution of NPs and AE-NPs in vitro and in vivo. (a) Flow binding diagram of CT26 and CAFs treated with DiI@NPs or AE-DiI@NPs for 1 h, respectively. Normal cells were served as negative controls. (b) Representative fluorescence microscopy images of CT26 and CAFs after treated using DiI-labeled NPs or AE-NPs for 1 h. (c) Living body images of CT26 tumor-bearing mice were acquired at fixed timepoint. The tumor area is marked in red circles. (d) Representative fluorescence images of excised tumors and major organs ex vivo. Heart (He), lung (Lu), spleen (Sp), kidney (Ki), liver (Li) and tumor (Tu). (e) The statistics of corresponding average radiation efficiency in five important organs and excised tumors. Scale bar, 100 μm. *P < 0.05, **P < 0.01