Fig. 5

Prophylactic effect of Agomir-122@M^mNP against frozen shoulder in a mouse model. A Schematic chart of model establishment, intervention, as well as sample harvest and observation. B Representative histological image of mouse shoulder joint capsule with different interventions, as illustrated by HE, Masson, and α-SMA staining (α-SMA in red and DAPI in blue). C Quantification of cell counts in joint capsule of different groups. D Quantification of joint capsule thickness of different groups. E α-SMA expression in joint capsule of different groups. F Passive ROM of the index shoulder of different groups. Scale bar: 200 or 100 μm. (n = 6). *p < 0.05; ***p < 0.001; ****p < 0.0001