Fig. 3
From: Smart osteoclasts targeted nanomedicine based on amorphous CaCO3 for effective osteoporosis reversal
Interfering effects of OAPLG on OCs formation. (a) Effect of different concentrations (μM) of ORO over time on BMMs activity. (b) Effect of different concentrations of nanocarriers on BMMs activity. (c) A scheme illustrating the experiment groups for OCs inhibition. (d) TRAP staining of OCs upon indicated treatments. (e) F-actin ring (green) and multiple nuclei (blue) and merged images of OCs. (f) The number of OCs and (g) the percentage of area occupied by OCs, based on the data from Fig. 3d. (h) Measurement of the F-actin ring area and (I) calculation of the percentage of nuclei within OCs, based on the data from Fig. 3e. Scale bar in all images: 100 μm, n = 3. *P < 0.05, **P < 0.01, ***P < 0.001; #P < 0.05, ##P < 0.01, ###P < 0.001 vs. OCs