Fig. 4
From: Smart osteoclasts targeted nanomedicine based on amorphous CaCO3 for effective osteoporosis reversal
Effect of OAPLG on OCs function and function-specific genes. (a) Microscopic images showing areas of OCs-mediated bone resorption after 10 days of specific treatment, scale bar 100 μm. (b) The percentage of bone resorption area by Fig. 3a. (c-f) Quantitative real-time PCR assessed that OAPLG downregulated expressions of osteoclast function effector genes (c) NFATc1, (d) Ctsk, (e) c-fos, (f) Atp6v0d2. (g) Western blotting analysis of expressed NFATc1, c-fos and Ctsk. n = 3, *P < 0.05, **P < 0.01, ***P < 0.001; #P < 0.05, ##P < 0.01, ###P < 0.001 vs. OCs