Fig. 3
Exosome functionalized by EGFR specific scFv-Lamp2b fusion protein. (A) Schematic diagram of scFv-lamp2b and lamp2b fusion protein. (B) Plasmid structure of the genes of the fusion proteins. (C) Western blot analysis of the fusion protein expression in exosomes and their parental cells. (D) Representative TEM imaging of exoscFv and exoctrl. Scale bar = 100 nm. (E) Size distribution of exoscFv and exoctrl. (F) Internalization of exoscFv and exoctrl visualized by confocal fluorescence imaging (Green: PKH76, Blue: DAPI). Scale bar = 20 μm